A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification
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منابع مشابه
A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification
BACKGROUND Linear regression of efficiency (LRE) introduced a new paradigm for real-time qPCR that enables large-scale absolute quantification by eliminating the need for standard curves. Developed through the application of sigmoidal mathematics to SYBR Green I-based assays, target quantity is derived directly from fluorescence readings within the central region of an amplification profile. Ho...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0017636